Yes. Even though buffer RDD in the RNase-Free DNase Set is optimized for on-column DNase digestion, the buffer is also well-suited for efficient DNase digestion in solution. Please note that the reaction must be cleaned up after the off-column DNase digest to remove the enzyme and buffer RDD, which will interfere with subsequent RT reactions.
hei,We are developing a new DNase, and thus need to test it in different buffers, including buffers that are commonly used. However, all these secret buffers makes life a bit hard. Can anyone tell me what the composition of Qiangens RDD buffer?
The diversity of the gut microbes in gut ecosystems can be categorized based on their morphological, structural, biochemical, and genetic properties. Gut microbes also have notable differences regarding cell wall structure and cell membranes, which enclose their cytoplasm and genomic contents. Harsh sample treatments can affect DNA quality, while a mild process may cause partial lysis of the microbial community (Baget al., 2016). Moreover, the unknown composition of the lysis buffer of kit-based methods presents different biases, drastically changing the results obtained from the same sample (Brookset al., 2015). Therefore, it is important to optimize cell lysis methods to obtain genomic DNA from all groups of microbes present in the gut. 2b1af7f3a8